Give the various steps involved in Glycolysis
The steps are as follows Glucose is phosphorylated in the presence of ATP, catalyzed by the enzyme hexokinase. Glucose – 6 – phosphate is changed into fructose – 6 – phosphate catalyzed by phosphohexose isomers. Fructose – 6 – phosphate is phosphorylated in the presence of ATP to form Fructose 1, 6 biphosphates. Fructose 1, 6 biphosphates is split into two molecules of triose phosphate one of 3 – phosphoglycerate dehyde and one of dihydroxyacetone phosphate, which is interconvertible. This reaction is catalyzed by phosphofructokinase. 3 – phosphoglyceraldehyde is oxidized to 1,3 biphosphoglycerate, with the reduction of NAD to NADH. Phosphoglycerate kinase catalyzes the formation of 3-phosphoglycerate to 1,3 biposphoglycerate and 1 molecule of ATP is produced directly (substrate phosphation). 3-phosphoglycerate is converted into 2-phosphoglycerate and then into phosphoenolpyruvate (PEP) PEP is converted into pyruvate along with the formation of one molecule of ATP directly. The enzyme pyruvate kinase catalyzes this step. The end products of glycolysis are 2 molecules of pyruvic acid + 2 NADH + 2ATP
