How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?
Hershey and Chase labored with bacteriophage and E.coli to show that DNA is the genetic fabric. They used specific radioactive isotopes to label DNA and protein coat of the bacteriophage. They grew a few bacteriophages on a medium containing radioactive phosphorus ( 32P) to become aware of DNA and a few on a medium containing radioactive sulphur ( 35S) to discover protein. Then, those radioactive labelled phages were allowed to contaminate E.coli micro organism. After infecting, the protein coat of the bacteriophage become separated from the bacterial mobileular with the aid of using mixing after which subjected to the method of centrifugation. Since the protein coat became lighter, it changed into determined withinside the supernatant at the same time as the inflamed micro organism were given settled on the bottom of the centrifuge tube. Hence, it turned into proved that DNA is the genetic cloth because it turned into transferred from virus to micro organism.
