What are the assumptions made during the calculation of net gain of ATP?
For theoretical calculation of ATP molecules, numerous assumptions are made, which can be as follows.(a) It is thought that diverse elements of cardio breathing along with glycolysis, TCA cycle, and ETS arise in a sequential and orderly pathway. (b) NADH produced at some point of the method of glycolysis enters into mitochondria to go through oxidative phosphorylation. (c) Glucose molecule is thought to be the most effective substrate at the same time as it's far assumed that no different molecule enters the pathway at intermediate stages. (d) The intermediates produced at some stage in respiratory aren't applied in another method.