Somaclones are obtained by [2009] (a) plant breeding (b) irradiation (c) genetic engineering (d) tissue culture
Option D. Somaclones is the term coined by Larkin and Scowcroft (1981) for plant variants obtained from tissue cultures of somatic tissues. Somaclonal variation leads to the creation of additional genetic variability and is beneficial in genetic engineering for selecting and crossing plants for creation of improved plants with desired traits So, the correct answer is 'Tissue culture'