Which of the following is not required in the preparation of a recombinant DNA molecules? a. Restriction endonucleases b. DNA ligase c. DNA fragments d. E.coli
Option D.E. coli is not required for preparation of a recombinant DNA molecule rather it may be required for the expression of recombinant DNA-molecule. Restriction enzymes and DNA ligases can be used to make a stable recombinant DNA molecule with DNA fragments that has been spliced together from two different organisms.
