The source of the restriction enzyme Hind III is (A) Escherichia coli RY 13 (B) Haemophilus influenzae RD (C) Bacillus amyloliquefaciens H (D) Streptomyces albus
Option B.Escherichia coli RY 13: Also known as E.coli, it is a typical bacteria found in the human intestine as a regular part of the gut. E.coli strains can survive in an open environment, hence, making it suitable for environmental benefits.EcoRI is isolated from E.coli strain RY13. E.coli is the most significant contributor as a host in the gene cloning mechanism. Haemophilus influenzae Rd: It is a gram-negative, facultatively anaerobic bacteria that belongs to the family Pasteurellaceae. HindIII is a type 2 specific site deoxyribonucleic restriction enzyme that is isolated from the Haemophilus influenzae Rd. It helps in the cleavage of DNA palindromic sequence in the presence of a cofactor.Haemophilus influenzae Rd: It is a gram-negative, facultatively anaerobic bacteria that belongs to the family Pasteurellaceae. HindIII is a type 2 specific site deoxyribonucleic restriction enzyme that is isolated from the Haemophilus influenzae Rd. It helps in the cleavage of DNA palindromic sequence in the presence of a cofactor.
