Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ? (1) Denaturation, Annealing, Extension (2) Denaturation, Extension, Annealing (3) Extension, Denaturation, Annealing (4) Annealing, Denaturation, Extension
Option 1. There are three steps done in PCR:- Step1:- Denaturation- The 2 DNA strands of template DNA separate from each other while heated to 92℃. Step 2:- Annealing- The primers anneal to the 3’ end of single strands of DNA. Step 3:- Extension- The primers are prolonged via way of means of DNA polymerase through the addition of nucleotides to shape whole strands of DNA. So, the correct sequence is:-Denaturation, annealing , extension.
