Do eukaryotic cells have restriction endonucleases? Justify your answer
Restriction endonuclease is a nuclease enzyme which recognizes a particular palindrome sequence, sticks to it and cuts the 2 strands of double helix at specific points through their sugar-phosphate backbone. No, eukaryotic cells do not tend to possess the enzyme restriction endonucleases. Restriction endonucleases are found in the bacterial cell and protect the cell from viral attack by disintegrating viral DNA. During disintegration of viral DNA, the bacterial genome is protected by having methylation of sensitive sites. The first restriction endonuclease discovered was Hind II (isolated from Haemophilus influenzae) and it was found to cut the DNA molecules at a particular site by recognising a specific sequence of 6 base pairs. These enzymes are used in the process of Recombinant DNA Technology for cutting of DNA at specific locations which result in formation of rDNA.
