Attempt titrating an amino acid against a weak base and discover the number of dissociating (ionizable) functional groups in the amino acid.
When an amino acid is titrated with weak base then its-COOH group also acts as weak acid. So it forms a salt with weak base then the pH of the resulting solution is near 7, so there is no sudden change. Number of dissociating functional groups are two, one is amino group (NH2) and another is carboxylic group ( – COOH). In the titration, amino acid acts as an indicator. Amino acids in solution acts as basic or acidic as situation demands. So these are also called amphipathic molecules.
