What are the various steps involved in glycolysis?
Following are the steps of glycolysis 1. The first step involved in the process of glycolysis is the conversion of D-glucose into glucose-6-phosphate. Hexokinase is the enzyme catalyst here. 2. The rearrangement of glucose 6-phosphate (G6P) into fructose 6-phosphate (F6P) takes place in this second step. 3. Fructose 6-phosphate is converted into fructose 1,6-bisphosphate using magnesium as a cofactor. 4. The enzyme Aldolase breaks fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (GAP). 5The enzyme triphosphate isomerase rapidly interconverts the molecules dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (GAP). The above five steps are considered as a preparatory phase 6.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) dehydrogenates and adds an inorganic phosphate to glyceraldehyde 3-phosphate, producing 1,3-bisphosphoglycerate. 7. A phosphate group from 1,3-bisphosphoglycerate is transferred to ADP to form ATP and 3-phosphoglycerate. 8. P from 3- phosphoglycerate from the 3rd carbon to the 2nd carbon to form 2-phosphoglycerate is relocated in this step. 9. A molecule of water is removed from 2-phosphoglycerate to form phosphoenolpyruvic acid (PEP) by an enzyme enclose. 10. A P from phosphoenolpyruvate (PEP) to ADP to form pyruvic acid and ATP.
