Describe briefly the followings: (a) Origin of replication (b) Bioreactors (c) Downstream processing
(a) Origin of replication -Origin of replication is described because the DNA series in a genome from wherein replication initiates. The initiation of replication may be both uni-directional or bi-directional. A protein complicated acknowledges the 'on' site, unwinds the 2 strands, and initiates the copying of the DNA. (b) Bioreactors - Bioreactors are big vessels used for the massive-scale manufacturing of biotechnology merchandise from raw materials. They offer surest situations to achieve the preferred product through presenting the most advantageous temperature, pH, vitamin, oxygen, etc. Bioreactors have an oxygen shipping system, a foam manage system, a PH, a temperature manipulate system, and a sampling port to gain a small quantity of lifestyle for sampling. (c) Downstream processing - Downstream processing is a technique of separation and purification of overseas gene merchandise after the finishing touch of the biosynthetic stage. The product is subjected to diverse approaches so as to separate and purify the product. After downstream processing, the product is formulated and is handed through numerous medical trials for high-satisfactory manage and different tests.
