How is the ELISA test carried out?
ELISA (Enzyme-Linked Immunosorbent Assay Test): It is a technique of detecting a very small amount of protein (antibody or antigen) with the help of enzyme peroxidase or alkaline phosphatase and stain-producing substrates like 5-aminosalicylic acid or orthophenylene diamine. The serum is sorbed to the surface of the ELISA plate. An antibody is specific to the antigen for diagnosis placed over an immobilized antigen. The spot is washed to remove the free antibody.
