Explain the post-transcriptional modifications the hn-RNA undergoes in eukaryotic cells.
In eukaryotes, the primary transcript is not functional due to the presence of exons and introns. Therefore it requires certain modifications to make it functional. First of all, introns are removed and the remaining exons are joined together in order by the process called Splicing. Then the hnRNA undergoes capping followed by tailing. In capping, methyl guanosine triphosphate is added to the 5’ end of hnRNA. In tailing 200 -300 adenylate residues are added to 3’ end in a template-independent manner. Now the hnRNA has been processed into mRNA.
