7. When We Do Liquid Extraction, What Is The Effect Of Adding 10%acid Or Any Base And How Do We Know Which Has To Be Added And Up To Which Concentration Or Micro Litre Level Can We Add Such Buffers? If Any Gel Formation Occurs At The Time Of Extraction, How Will It Affect The Analysis? Shall We Continue The Extraction With The Same Or Should We Drop That Method?
The idea of adding acid/base is to change the lipid (and therefore organic solvent) solubility of the components you want to extract. For example, a fatty acid is more soluble in water as a salt (and, thus, in basic conditions) whereas it is largely insoluble in water in its uncharged state (in acid conditions). So adding acid to the solution of fatty acid salt in water will render it water-insoluble and, thus, move it from the water to the organic solvent.
