Which one of the following statement is not true regarding gel electrophoresis technique? A. The separated DNA fragments are stained by using ethidium bromide B. The presence of chromogenic substrate gives blue coloured DNA bands on the gel C. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light D. The process of extraction of separated DNA strands from gel is called elution
Option B Agarose gel electrophoresis is mainly used to separate the DNA molecule on the agarose gel matrix. The negatively charged molecules of DNA are loaded as a sample on the deeply carved wells on the gel. The loaded sample is induced by a strong electric field which leads to the separation of DNA on the basis of mass and the charge on the DNA. As all the DNA molecules have the same charge so they get aligned on the basis of mass. The smaller DNA molecules moves faster and get aligned at the top with the larger segment at the bottom.
