25.A good cloning vector should have all except:- A. Origin of replication to start replication B. Many recognition sites for single restriction enzyme C. High copy number D. Selectable marker
Option B All cloning vectors must have at least one cloning site (a specific DNA sequence that is recognized and cut by a restriction endonuclease), where the foreign DNA is inserted. Three classes of restriction enzymes bind to DNA at the recognition sequence and hydrolyze the phosphodiester bond on both strands of DNA.
