52.Which of the following is true regarding DNA fragments during gel electrophoresis? A. Larger the fragment size, farther it moves B. Osmium chloride can be used for visualization during gel electrophoresis C. DNA Fragments migrate towards the anode D. Bees wax can be used as matrix
Option C. The smaller the fragment size, the farther it moves Gel electrophoresis is a technique for separation of DNA strands of different sizes using electric current. Agarose gel acts as a sieve to selectively allow movement of DNA based on size. The smaller fragments will move faster compared to larger fragments. DNA molecules are negatively charged due to the phosphate backbone.Positive and negative electrodes are present on the two sides of the gel. DNA sample is added to the wells near the negative electrode. When the current is passed through the gel, the DNA starts moving towards the positive electrode. Here, the smaller fragments moves quickly and reaches the farther end while the larger fragments trail behind. The DNA containing ethidium bromide is observed under UV lights.
