In probe the nucleic acid which is tagged with a. A single stranded RNA b. A single stranded DNA c. A double stranded DNA d. Both (a) & (b)
Option D.Probe is a stretch of oligonucleotides of DNA or RNA generally containing 15-20 nucleotides. It is single-stranded and complementary to the region of DNA or RNA depending on the template. It is usually labelled with radioactive phosphorus or fluorescent dyes for detection in autoradiography. Probes hybridise to their complementary counterparts and help in the detection of specific sequences of DNA or RNA in the sample.A double stranded DNA cannot be used as a hybridisation probe as it is already present in pairs and cannot form hydrogen bonds with the DNA to be identified.
