During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ? (1) Annealing (2) Extension (3) Denaturation (4) Ligation
Option 3. During the process of gene amplification using PCR , If very high temperature is not maintained in the beginning Denaturation process will be affected. In order for the primers to bind to the target region and start an extension, the double-stranded template DNA should first be separated into single strands all through the preliminary denaturation level of PCR. Commonly, the primary denaturation segment is done at 95° to 98 °C for one to 3 minutes. This step's period and temperature can alternate primarily based totally at the sort of template DNA used and the buffer's salt content.
