Explain briefly (b) Restriction enzymes and DNA
Restriction enzymes are molecular scissors utilized in molecular biology to cut DNA series from a particular site. It performs a significant part in gene manipulation. The enzymes identify a certain six-box pair sequence called the recognition sequence and cut the sequence at a particular site. A restriction enzyme is characterized into two kinds − (i) Exonuclease − It is a kind of restriction enzyme that eradicates the nucleotide from either 3’ or 5’ ends of the DNA molecule. (ii) Endonuclease − It is a kind of restriction enzyme that cuts within the DNA at a certain position. This enzyme functions as a significant tool in genetic engineering. It is generally utilized to cut the sequence to attain DNA fragments with sticky ends, which are later united by DNA ligase. Final answer A restriction enzyme is a DNA-cutting enzyme that identifies certain sites in DNA. Several restriction enzymes create staggered cuts close to or at their recognition sites, creating ends with a single-stranded overhang. If two DNA molecules contain matching ends, they can be united by the enzyme DNA ligase.
